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 A question about Fleas (Siphonaptera)
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| pwalter |
Posted on 09-06-2009 13:21
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 Member Location: Posts: 3555 Joined: 06.11.08 |
HI! I finally managed to catch a flea of a dog - first time in my life I see this insect order. Can anyone suggest how to examine it? Should I use NaOH to make it transparent with microscope? Should I dissect genitalia? How? It's some 2,5 mm, so not too small. |
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| phil withers |
Posted on 09-06-2009 14:07
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Member Location: Posts: 521 Joined: 04.03.08 |
Clear with NaOH then mount in DMSO or on a slide in...whatever you like. You cannot dissect the genitalia, they are internal. |
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| pwalter |
Posted on 09-06-2009 14:23
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Member Location: Posts: 3555 Joined: 06.11.08 |
Thank You! |
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| Paul Beuk |
Posted on 09-06-2009 14:35
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 Super Administrator Location: Posts: 19208 Joined: 11.05.04 |
DMSO?
Paul - - - - Paul Beuk on https://diptera.info |
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| Tony Irwin |
Posted on 09-06-2009 15:55
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 Member Location: Posts: 7168 Joined: 19.11.04 |
Dimethyl sulfoxide
Tony ---------- Tony Irwin |
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| pwalter |
Posted on 09-06-2009 16:02
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Member Location: Posts: 3555 Joined: 06.11.08 |
I was suprised to hear DMSO - at the university we use it for cryoprotection of mammalian cells stored in liquid nitrogen. |
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| phil withers |
Posted on 09-06-2009 21:24
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Member Location: Posts: 521 Joined: 04.03.08 |
I meant DMHF - sorry (dimethyl hydantoin formaldehyde, before Paul asks). DMSO just slipped out...must have been thinking of my old life there... |
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| Paul Beuk |
Posted on 10-06-2009 08:33
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Super Administrator Location: Posts: 19208 Joined: 11.05.04 |
Is it a mounting agent or something to store it in?
Paul - - - - Paul Beuk on https://diptera.info |
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| phil withers |
Posted on 10-06-2009 09:03
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Member Location: Posts: 521 Joined: 04.03.08 |
Mounting agent (I use it for genitalia...not mine though). It is crystalline, and when added to water forms a clear liquid which dries to transparent: the meniscus formed helps by magnifying too. |
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but don't see the image in the post.
