Thread subject: Diptera.info :: How to preparate Turbellarians?

Posted by pwalter on 09-10-2009 21:22
#1

Does anyone have any info on how to make microscope-preparations from Turbellarians? Thanks!

Posted by Kahis on 09-10-2009 22:21
#2

Of course I do. Who doesnt Smile

The answer depends on the indended use. For identification "it is necessary to have a combination of drawings or photographs and notes from life, a whole mount where possible, and sections for anatomical reconsitruction"

"fixation for light microscopy is achieved with most standard fixatives. Fr large worms formalin (around 8% by volume) is suitable.

"for most microturbellarians there is little point in making whole mounts except to preserve and reveal hard structures for fixation often renders most anatomical details unobservable"

Quotes from Higgings & Thiel, "Introduction to the Study of Meiofauna".

Edited by Kahis on 09-10-2009 22:28

Posted by pwalter on 09-10-2009 22:54
#3

Thank You a lot! I have 5-7 mm long ones, so largers. Do You know what to do with them after fromalin? Can I mount them on slide?